2 edition of Gene expression in cardiac surgery found in the catalog.
Gene expression in cardiac surgery
Igor E. Konstantinov
Written in English
Ischemia and reperfusion (IR) invariably occurs during cardiac surgery. However, the transcriptional regulation of the myocardial and leukocyte genome in IR injury is incompletely elucidated. The primary hypothesis of this thesis is that IR injury occurring during cardiac surgery results in rapid induction of a specific pattern of gene transcription. The secondary hypothesis concerns the genomic and physiologic responses to protective brief periods of IR applied to the tissue located remotely from the heart (i.e., by a remote ischemic preconditioning (rIPC) stimulus). Gene expression was assessed primarily using microarray technology. In an initial study, we observed up-regulation of genes with possible cytoprotective functions in neonatal myocardium during ischemia. Global human myocardial gene expression during intra-operative IR injury was determined in a second study. A series of studies were then designed to examine the genomic and functional responses to a clinically-relevant rIPC stimulus. As leukocytes play a key role in IR injury, we identified the global genomic changes in human leukocytes following the rIPC stimulus. We then examined myocardial genomic responses in a mouse model, and, finally, demonstrated a major protective effect of rIPC in a porcine model of cardiopulmonary bypass (CPB) and intra-operative IR injury. Based on these data we applied the rIPC clinically with encouraging preliminary results.Overall the results of these studies indicate that intra-operative myocardial IR injury results in rapid induction of gene expression, and that this genomic response appears to be age-specific and can be modified by rIPC.
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RNAs and Gene Expression Predicting Postoperative Atrial Fibrillation in Cardiac Surgery Patients Undergoing Coronary Artery Bypass Grafting by Muhammad Shuja Khan 1, Kennosuke Yamashita 1,2, Vikas Sharma 3, Ravi Ranjan 1,2,4 and Derek James Dosdall 1,2,3,4,*. Abstract: Nitroglycerin exerts a direct myocardial anti-ischemic effect even in the state of vascular nitrate tolerance. To examine the potentially diverse molecular responses in vascular and cardiac tissues, we investigated the gene expression profile of the heart and the aorta by DNA microarray in male Wistar rats that were previously made tolerant to the vascular effects of nitroglycerin.
Gene expression profiling is the measurement of the activity of thousands of genes at once, to create a picture of cellular functions. Gene expression provides wider dynamic range and improved. A subset of the MI-induced and sham rats received mechanical unloading using a syngeneic heterotopic transplanted heart at 6 weeks, followed by assessment of cardiac gene expression at 8 weeks. 2 At 6 weeks post-MI, the LV ejection fraction in the MI-induced rats had decreased from 75% to about 25%, along with a significant increase in the LV.
Background: In patients with coronary artery disease (CAD), epicardial adipose tissue (EAT) has been shown to express increased levels of inflammatory cytokines (IL-1β, IL-6, MCP-1, TNFα) and decreased levels of anti-inflammatory and cardioprotective adipokines. However, it is not known whether or not inflammation in EAT is a primary cause or a secondary response to : Nancy Lee, Sarah M. Nicoloro, Juerg R. Straubhaar, Melinda Darrigo, Stanley Tam, Michael P. Czech, T. Genome-wide gene expression profiling is an extensively used strategy for discovering new potential biomarkers for diagnosis/prediction of disease severity [1,2] and identification of novel drug targets .Transcriptome analysis has been applied successfully to numerous complex diseases including cardiovascular disorders [4,5].Coronary heart disease (CHD) is one of the major causes of heart Cited by:
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Cardiac surgery with gene therapy clinical trials brought many positive hints that gene transfer may eventually be effective. However, it is a best practices tenet to conduct clinical trials under double-blinded, placebo-controlled, randomized conditions with a sufficient number of patients.
This is the only way to answer the question of how Cited by: Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression.
The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Effective gene therapy for heart failure has not yet been achieved clinically. The aim of this study is to quantitatively assess the cardiac isolation efficiency of the molecular cardiac surgery with recirculating delivery (MCARD ™) and to evaluate its efficacy as a means to limit collateral organ gene expression.
10 14 genome copies (GC) of recombinant adeno-associated viral vector 6 Cited by: 7. The global myocardial stress response during cardiac surgery has not been systematically studied, nor is it known whether the response of the neonatal myocardium is intrinsically different from that of older children.
To determine the age-related molecular basis of this response, we conducted microarray-based differential gene expression profiling on right ventricular tissue samples acquired Cited by: Inherited cardiac conditions are associated with many different genes, with most Gene expression in cardiac surgery book gene variants being unique familial variants 1, and therefore, seen infrequently.
2 Additionally, panels currently used in clinical research can miss causal gene variants implicated in inherited cardiac conditions (ICCs). Gene expression profiles in children undergoing cardiac surgery for right heart obstructive lesions 1 Read at the Eighty-third Annual Meeting of The American Association for Cited by: Investigation with miRa, antimiRa and AdSRF-VP16 experiments in H9c2 cardiac cells demonstrated that: 1)-miRa acts as a repressor of SRF and CTGF expression; 2)-a simultaneous overexpression of SRF by AdSRF-VP16 and inhibition of miRa by a specific antimiR increase CTGF expression; 3)-miRa overexpression can block the.
Gene Expression Tests for Cardiac Indications Page 3 of 6 UnitedHealthcare Oxford Clinical Policy Effective 11/01/ ©, Oxford Health Plans, LLC evidence. This information serves to provide health care providers, policymakers, and others to distinguish genetic tests that are safe and useful (CDC, ).
Coronary Artery Disease. Technologies used for genetic testing of cardiac syndromes and coronary artery disease can vary.
Tests can include, but are not limited to, those that evaluate variations in the genes, such as chromosome microarray and next generation sequencing (NGS), as well as others that assess the gene products, such as gene expression arrays and.
Naoyuki Kimura, Kyoko Futamura, Mamoru Arakawa, Naoko Okada, Fabian Emrich, Homare Okamura, Tetsuya Sato, Yasuhiro Shudo, Tiffany K. Koyano, Atsushi Yamaguchi, Hideo Adachi, Akio Matsuda, Koji Kawahito, Kenji Matsumoto, Michael P.
Fischbein, Gene expression profiling of acute type A aortic dissection combined with in vitro assessment, European Journal of Cardio-Thoracic Cited by: 8.
The study results suggest that modifying gene expression may provide a path to preventive treatments for heart failure. "In terms of disease progression, heart failure is the end stage. In the short-term this is accomplished by the Frank-Starling mechanism, but in the long-term or after repeated increases in load, the heart adapts through an increase in myocardial mass (Fig.
) frequently involving changes in cardiac gene expression. 8,9,11 The resulting hypertrophy helps compensate for excessive loading through an increase Cited by: Quantitative assessment of mononuclear-cell gene expression in peripheral-blood specimens has been explored as a method for detecting the rejection of a cardiac transplant.
13,14 This approach has Cited by: Objectives We evaluated ankyrin repeat domain 1 (ANKRD1), the gene encoding cardiac ankyrin repeat protein (CARP), as a novel candidate gene for dilated cardiomyopathy (DCM) through mutation analysis of a cohort of familial or idiopathic DCM patients, based on the hypothesis that inherited dysfunction of mechanical stretch-based signaling is present in a subset of DCM by: To determine the role of MeCP2 in the regulation of cardiac gene expression in vivo,> we performed qRT-PCR analyses of cardiac genes, using total RNA prepared from whole heart taken from 6- Cited by: We examined the effects of cardiothoracic surgery, including cardiopulmonary bypass and controlled mechanical ventilation, on messenger RNA gene expression in human diaphragm.
We hypothesized that genes responsible for stress response, redox regulation, protein turnover, energy metabolism, and contractile function would be altered by Cited by: Gene expression in cardiac tissues from infants with idiopathic conotruncal defects Article (PDF Available) in BMC Medical Genomics 4(1):1 January with 51 Reads How we measure 'reads'.
Heatmap of age-related gene changes in the Drosophila heart with associated Gene Ontology terms and miR binding site enrichment.
5-week-old yw and yw x GMH5 fly hearts show significant up-and down. Gene expression proﬁles in children undergoing cardiac surgery for right heart obstructive lesions Igor E. Konstantinov, MDa John G. Coles, MDa Cathy Boscarino, MSca Mark Takahashi, PhDb Jason Goncalves, MScb Julia Ritter, MDa Glen S.
Van Arsdell, MDa Background: The global myocardial stress response during cardiac surgery has not been systematically studied, nor is it known whether the. Myocardial gene expression of the CRF family of ligands and receptors. Expression of CRFR1, CRFR2α, CRF, Ucn1, Ucn2, and Ucn3 was detected within the LV of the myocardium of heart donors and heart failure patients ().In heart donors, CRFR2α was the most abundantly expressed average, the expression levels of CRFR1 and CRFR2β were markedly lower than Cited by: 6.
Mohamed T. Ghorbel, Gianni D. Angelini and Massimo Caputo (February 3rd ). Gene Expression Profiling - A New Approach in the Study of Congenital Heart Disease, Front Lines of Thoracic Surgery, Stefano Nazari, IntechOpen, DOI: / Available from:Author: Mohamed T.
Ghorbel, Gianni D. Angelini, Massimo Caputo. Gene Expression Analysis of Cardiovascular Diseases: Novel Insights to Biology and Clinical Applications David Seo, Geoffrey S.
Ginsburg, Pascal J. Goldschmidt-Clermont Microarray technology gives researchers the ability to survey the expression levels of thousands of genes simultaneously and is a powerful tool for studying complex cardiovascular by: In adult and pediatric cardiac surgery, the use of cardioplegic arrest and cardiopulmonary bypass (CPB) remains a common requirement.
Although this approach allows for a quiescent and bloodless surgical field, myocardial reperfusion and separation from CPB is associated with hemostatic and hemodynamic sequelae that can complicate the early postoperative course ().